Richardot M, Debroas D, Thouvenot A, et al.

Influence of cladoceran grazing activity on dissolved organic matter, enzymatic hydrolysis and bacterial growth
J PLANKTON RES 23 (11): 1249-1261 NOV 2001

Abstract:
To assess the influence of grazing by cladocerans on dissolved organic matter (DOM), glycolytic and proteolytic activities and bacterial growth were measured by in situ incubation of lake water from the epilimnion of an oligotrophic reservoir in three different treatments: in absence of zooplankton, and in presence of zooplankton (natural abundance and concentrated four-fold). These experiments were conducted at two periods in the succession of plankton populations (May and June 1998), that differed in the quality of the prey ingested (Eudorina sp. compared to Cryptomonas sp. and Rhodomonas sp.) and their grazing intensity (31.8 +/- 2.2 mug C l(-1) day(-1) compared to 10.2 +/- 0.5 mug C l(-1) day(-1)). A systematic increase in bacterial biomass was measured in the treatments containing the highest zooplankton concentrations. The DOM concentrations produced in situ showed few significant differences between the three treatments, but the assimilation of DOM was higher in the presence of zooplankton than in their absence. These results show that the influence of cladocerans on the DOM was more of a qualitative than a quantitative nature. The protein compounds derived from the grazing activities of metazoans seem to be a major nutrient source for growth for bacteria (r = 0.81, P < 0.05). In this study, the highest hydrolytic activities were recorded in the presence of high concentrations of metazoan zooplankton. However, the processes that regulated these activities differed between the two experimental dates (repression compared to enzyme stimulation). Grazing activities could lead to an increase in phytoplanktonic excretion during the growth phase, and therefore the production of low molecular weight compounds that are easily assimilated by the bacterial plankton.

Coleman AW

Biogeography and speciation in the Pandorina/Volvulina (Chlorophyta) superclade
J PHYCOL 37 (5): 836-851 OCT 2001

Abstract:
Mating affinity, nuclear rDNA internal transcribed spacer (ITS) sequence, and geographic distribution of more than 100 isolates of Pandorina,Volvulina, and Yamagishiella were determined. Comparative analysis of ITS sequences reaffirmed the unity of Pandorina morum and its separation from all other species of Volvocaceae except those of Volvulina. This latter genus, represented by four species, appears to represent only morphological variants of several P. morum subclades. The P. morum clade (including also additional species such as P. smithii and P. colemaniae) encompasses an evolutionary span, as determined by comparison of ITS, greater than either of the multispecies genera Gonium or Eudorina. There are at least 30 Pandorina/Volvulina syngens, sexually isolated groups, so far as can be determined, among the current collection of strains. In addition, as in other volvocacaean genera, two clones are homothallic, capable of forming zygotes within a genetic clone. The existence of so many syngens suggests that considerable evolutionary diversification of the genes controlling gamete compatibility and intercross survival has occurred, unaccompanied by significant morphological change. Within each syngen, genetic distance increases with geographic distance between collection sites. At least half of the isolates studied must have been introduced northward since the Pleistocene. Although we probably know more about characters in this group of algae than any other algal group, in part because they are so easy to culture, we are still largely ignorant of what circumscribes their niche in nature. The study of all these organisms, distributed throughout the world presumably by the activities of shorebirds, emphasizes the evolutionary role of mating genes in the inevitable formation of new genetically isolated subclades in these eukaryotes and provides initial data on their rate of appearance versus their rate of distribution over the earth.

Nozaki H, Krienitz L

Morphology and phylogeny of Eudorina minodii (Chodat) Nozaki et Krienitz, comb. nov (Volvocales, Chlorophyta) from Germany
EUR J PHYCOL 36 (1): 23-28 FEB 2001

Abstract:
Morphology, sexual reproduction and phylogeny of a colonial green alga collected from Germany were studied in culture. Light and electron microscopy of the gelatinous (extracellular) matrix of vegetative colonies, the absence of obligately somatic cells, and the anisogamous sexual reproduction with sperm packets in this alga indicated that if: is assignable to the genus Eudorina. This German alga was similar to E. elegans Ehrenberg in its multiple pyrenoids of nearly identical size and almost identical sized vegetative cells in the colony, but differed from it in having a prominent tubular structure (flagellar sheath) surrounding each flagellum in the gelatinous matrix of the vegetative colonies. Sexual reproduction was homothallic and dioecious. On the other hand, the vegetative morphology agreed well with that of Pandorina minodii Chodat with regard to multiple pyrenoids and the prominent flagellar sheaths. Thus, a new combination, Eudorina minodii (Chodat) Nozaki et Krienitz, is proposed. Molecular phylogenetic analyses of the rbcL-atpB gene sequences from the colonial Volvocales reserved that E. minodii and several heterothallic strains of E, elegans constituted a robust clade. Therefore, prominent flagellar sheaths and homothallic sexual reproduction in E. minodii may be derived characters that evolved recently within the clade.